Coding

Part:BBa_K4382008:Design

Designed by: UZAIR MOTIWALA   Group: iGEM22_IISER_Bhopal   (2022-10-08)


Pectin Methyl Esterase (Pme)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 48
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 48
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 192
    Illegal BamHI site found at 1080
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 48
    Illegal NgoMIV site found at 343
    Illegal AgeI site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is codon optimized for bacillus


Source

Ralstonia solanacearum

References